Authors: Rahmah Hayati, Mohammad Basyuni, Diana Chalil

Abstract. Ganoderma boninense is the most severe pathogen of Elaeis guineensis in Sumatra and Sulawesi, Indonesia, owing to the fact that it causes upper or basal stem rot disease. This study aimed to determine the genetic diversity, sequence and bioinformatics of E. guineensis, Borassus flabellifer, Hevea brasiliensis and Cocos nucifera. As many as 49 individuals of G. boninense from six populations were analyzed using the four SSR marker loci. The genetic structure was carried out by the GenAlEx ver 6.502 software. The sequences compared database with the BLASTX program. Physico-chemical, possible transfer peptide, and subcellular localization relationships were analyzed using the bioinformatics methods. The clustering UPGMA and phylogenetic were generated using MVSP ver. 3.22 and CLUSTAL W ver. 1.83 software. Results of the analysis showed that the observed heterozygosity (Ho) was 0.32. The two loci that revealed polymorphism with a polymorphic information content (PIC) value was 0.61 (KT124397 and KT12440). Further, the distribution of the BLASTX analyses indicated that the isolates were more similar to the Transcription factor (G. sinense strain ZZ0214-1) homologs in some other fungi. The potential peptides prediction in Ganoderma was similarly localized to the chloroplasts and mitochondria (0.1). The two subcellular principal predicted in the cytoplasm and mitochondria localization were stable (2.0–8.5). They were UPGMA clustered into two distinct geographical distributions. The isolates from the North Sulawesi Province have a genetic correlation close to the isolates from the North Sumatra Province. However, the phylogenetic clearly separated the G. boninense and G. sinense.

Sumber : https://www.fspublishers.org/Issue.php?y=2020&v_no=23&categoryID=907